Smart Hybrid Materials (SHMs)
مختبر شمس لعلوم النانو
Self-Assembled Nanomaterials for Biomedical, Sensing and Separation Applications

Publications 2016

Dissociation Coefficients of Protein Adsorption to Nanoparticles as Quantitative Metrics for Description of the Protein Corona: A Comparison of Experimental Techniques and Methodological Relevance

Hühn, J.; Fedeli, C.; Zhang, Q.; Masood, A.; del Pino, P.; Khashab, N. M.; Papini, E.; Parak, W. J. Dissociation Coefficients of Protein Adsorption to Nanoparticles as Quantitative Metrics for Description of the Protein Corona: A Comparison of Experimental Techniques and Methodological Relevance. The International Journal of Biochemistry & Cell Biology 2016, 75, 148-161
Hühn, J.; Fedeli, C.; Zhang, Q.; Masood, A.; del Pino, P.; Khashab, N. M.; Papini, E.; Parak, W. J.
Protein corona, dissociation constant, Hill model, nanoparticles
2016
Protein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process is defined by a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein–nanoparticle interaction for quantitative evaluation and objective literature comparison.