Smart Hybrid Materials (SHMs)
مختبر شمس لعلوم النانو
Self-Assembled Nanomaterials for Biomedical, Sensing and Separation Applications

Publications 2017

Thermoresponsive pegylated bubble liposome nanovectors for ef cient siRNA delivery via endosomal escape

Kholod Alamoudi, Patricia Martins, Jonas G Croissant, Sachin Patil, Haneen Omar;  Niveen M Khashab. Thermoresponsive pegylated bubble liposome nanovectors for ef cient siRNA delivery via endosomal escape​​. Nanomedicine201712​​​​​​​​​​​​​​​​​​​​
K. Alamoudi, P. Martins, J.G. Croissant, S. Patil, H. Omar, N.M. Khashab
bubble generation, endosomal escape, gene silencing, lipoplex, liposome, multidrug-resistant cell, nanomedicine, siRNA delivery
2017
Aim: Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy. Methods: A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing. Results: The siRNA- liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was con rmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes. Conclusion: The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an ef cient gene silencing.​


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