Smart Hybrid Materials (SHMs)
مختبر شمس لعلوم النانو
Self-Assembled Nanomaterials for Biomedical, Sensing and Separation Applications

Publications 2018

Endosomal escape and delivery of CRISPR/Cas9 genome editing machinery enabled by nanoscale zeolitic imidazolate framework

Alsaiari, Shahad K.; Patil, Sachin; Alyami, Mram; Alamoudi, Kholod O.; Aleisa, Fajr A.; Merzaban, Jasmeen S.; Li, Mo; Khashab, Niveen M. Endosomal escape and delivery of CRISPR/Cas9 genome editing machinery enabled by nanoscale zeolitic imidazolate framework. Journal of the American Chemical Society (2018), 140(1), 143-146​
Alsaiari, Shahad K.; Patil, Sachin; Alyami, Mram; Alamoudi, Kholod O.; Aleisa, Fajr A.; Merzaban, Jasmeen S.; Li, Mo; Khashab, Niveen M.
CRISPR Delivery, protein Delivery, ZIFs
2018
​CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to genetic diseases. It has, however, a double delivery problem owning to the large protein size and the highly charged RNA component. In this work, we report the first example of CRISPR/Cas9 encapsulated by nanoscale zeolitic imidazole frameworks (ZIFs) with a loading efficiency of 17% and enhanced endosomal escape promoted by the protonated imidazole moieties. The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (CC-ZIFs) is further verified by knocking down the gene expression of green fluorescent protein by 37% over 4 days. The nanoscale CC-ZIFs are biocompatible and easily scaled-up offering excellent loading capacity and controlled codelivery of intact Cas9 protein and sgRNA.