The salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present study. In this paper, we report the incompatibility of 3D printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification. The compatibility of the material to build microfluidic devices was evaluated in three different ways (1) determining if the UV cured resin inhibits the polymerase chain reaction (PCR) i.e., testing devices for PCR compatibility and (2) observing agglutination complex formed on the surface of the UV cured resin when anti-CRP antibodies and Creactive protein (CRP) proteins were allowed to agglutinate (3) by culturing human embryonic kidney cell line (HEK) cells and testing for its attachment and viability. The study showed that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells while the others were used after treating the surface. This study finds importance in building Lab-on-chip/Micro Total analysis systems (μTAS) and organ-on-chip devices.